NEWS

Dying fishes in the Cavaliere river

The dying fish and the numerous carcasses found in the Cavaliere river by FIPSAS in recent days represent an alarm signal for possible criticalities in the waterways of the Isernina area. It will be the task of the Isernia Zooprophylactic Institute to establish the causes of fish deaths, but it is easy to think of…

Scientific paper in MDPI – Fishes: Cryobank of Mediterranean Brown Trout Semen: Evaluation of the Use of Frozen Semen up to Six Hours Post-Collection

The main objective of our project is to safeguard the native trout of Molise and the habitats they inhabit, but it is still important that the results obtained are shared with the scientific community through the publication of articles in international journals. This constant activity is essential so that the model we are applying in…

Cleand the reproductive sites at the sources of Biferno river

We have cleaned up the breeding sites at the sources of the Biferno River from several quintals of urban waste. It was not uncommon to see trout trying to dig their nest in the gravel between a glass bottle, a can and a tire. Having freed the gravel from much of the waste has certainly…

BUILT THE PASSAGE FOR FISH ON THE RIO SANTA MARIA

In these hot days of midsummer, the project does not stop: in fact, the construction of the fish passage near a bridle on the Rio Santa Maria, in Bojano has just finished.   The study of the movements of native trout in the Biferno basin has shown us that large breeders need the greatest freedom…

Completed the works on the Rio Caprionero

The Caprionero stream is an important tributary of the Volturno river, where native trout usually go to reproduce. As is known, near Sant’Eusanio, it was interrupted in its continuity by a waterfall of about one meter jump and by an artificial section in reinforced concrete box without any shelter for the ascent of the fish…

NatSalMo to the children

Last Friday, the second NatSalMo appointment dedicated to young people was held at the Galassport sports center in Campobasso, as part of the summer camps organized by the structure’s educators. During the meeting, through the projection of images and the game, the objectives of the project were first explained, after which various issues were addressed…

Nat.Sal.Mo. in local schools

The Covid-19 pandemic and the various consequent lockdowns had kept us away from schools, forcing us to completely reshape the training offer that we had planned at the beginning of the project. But we managed to do something, even if with great difficulty, and the result makes us very proud. On April 16, in fact,…

Scientific Paper published on MDPI – Animals: Genotyping of Two Mediterranean Trout Populations in Central-Southern Italy for Conservation Purposes Using a Rainbow-Trout-Derived SNP Array

Simple Summary Mediterranean trout is one of the most threatened freshwater fish at risk of extinction. A complex pattern of climatic and anthropogenic pressures has dramatically compromised its biodiversity. In particular, the introduction of non-native trout represents one of the most important threats with negative impact on intraspecific diversity of native populations. The introgressive hybridization…

World Environment Day 2021

The theme of this year’s Environment Day is the “Restoration of Ecosystems”, with the aim of preventing, stopping and reversing the damage inflicted on the planet’s ecosystems, thus trying to move from the exploitation of nature to its healing. And with World Environment Day 2021, the United Nations officially launches the United Nations Decade for…

The mimicry of the trout of Molise

The river habitat is pure dynamism. Nothing stands still. The play of light and shadow, of dazzling reflections and shady branches, creates the dynamic background within which the trout live. And the speckling and zebra pattern of the Molise trout is not only to be read in a static way, because depending on the colors…

LIFE NatSalMo at Agri_For_Food

Agri_For_Food 2021, the scientific orientation and study initiative conceived by Unimol for the promotion of degree courses in “green sciences”, ended yesterday with the awarding of innovative ideas in the agri-food and forestry sectors, Now in its third edition, on 12 May it also hosted our project, objectives, activities, and the results of which were…

Males guarding reproduction bed at the sources of the Biferno

At the sources of the Biferno, among the tufts of aquatic plants, the trout dig their own beds of scrub, bringing to light the white gravel. The individuals seen in the video are mostly males manning the breeding site and fighting for the best position while waiting for the female. The female is present and…

Searching for male breeding specimens

Sometimes we go in search of reproducers through the electro-fishing. This happens, for example, in the side courses of the Volturno where it is not possible to anchor fixed capture stations due to the high risk of floods. During these activities we go both in search of marked specimens and specimens to tag, in order…

The Lorda stream in Castelpizzuto

Between the end of February and the beginning of March, the eradication of alien trout and subsequent sowing of 8,000 eggs and newly hatched larvae of native Mediterranean trout took place in the upper section of the Lorda stream near the Municipality of Castelpizzuto. The Municipality of Castelpizzuto has decided to actively support our project,…

Fertilization by doses of frozen semen

The material stored in our cryobank is always available and ready for use. It allows us to always have the semen of dozens of genotyped native males available to fertilize the eggs of the females captured in the fixed stations. In the video we are in one of the side stations of the Volturno and…

Biferno – sowing 2021

At the beginning of March we began to sow the Biferno eggs incubated in the Oratino hatchery. The first 15 nests were built on the Rio Freddo, at the sources of the Biferno di Pietre Cadute and at the sources of the Rio Santa Maria. Inside them we sowed the first 10,000 embryonated eggs. In…

Rocchetta: Work in progress

Despite the pandemic and the entry of Molise into the red zone, the works to adapt the facilities of the Rocchetta a Volturno plant continue in maximum safety. In the pre-existing external tanks, partitions are being built to allow a rational use of the structures, especially with an eye to the future, when these places…

Trout “eyed” egg observed under a digital microscope

At the Rocchetta a Volturno hatchery, during the EASME monitoring visit on 11 and 12 February, we observed through a digital microscope an egg that we had fertilized in early January with the cryopreserved seed. When fertilization is successful, at a certain point of the incubation those two black points appear, which correspond to the…

The cryobank on Dire.it

The press release on the first European cryobank of Mediterranean trout seed created by Unimol thanks to LIFE NatSalMo had considerable success, not only in the traditional press (see the press review), but also in local and national news. At the link, one for all, the Tg di Dire.it, a national press:  

The cryobank and the protocol for cryopreservation of native Mediterranean trout semen: an excellence of the NatSalMo project

The cryobank of the semen proved to be an effective strategy to protect the biodiversity of some fish species and the cryopreservation protocol, originally developed for farmed salmonids, was tested for the first time on the sperm of wild native trout that populate the Molise rivers by the scientific project staff. Thus was born, in…

January 2021 on the Volturno river

January is the main month for spawning in the Volturno River and its sides. Females mature their eggs, approach breeding sites, build nests and deposit thousands of small yellow eggs there. The color is due to the yolk which will be the nourishment first for the embryo and then for the newly hatched larvae (the…

The circles of deposition of the scales in the trout

To know how to protect a species and manage the human activities connected to it, it is important to know its biological characteristics, dynamics and behaviors. The age, the growth rate of the trout and therefore the length reached at the first reproduction are decisive parameters. To estimate the age of the fish, we can…

The semen collection on the Volturno: season ’20/’21

In December, the semen collection of the native Mediterranean trout individuals on the Volturno River began. Here in particular the staff is on the Lorda, here are the first images:

Searching for males on Volturno river – December 2020

The project technical staff spent the whole month of December on the Volturno looking for native Mediterranean trout males for sampling and taking the new semen to be used for the new upcoming fertilizations and for the cryobank. In search of males on the Volturno:

Installation of the cages in the Volturno river

Our technical-scientific staff has spent the last few weeks on the Volturno to install the cages necessary for the capture of the males going up to the breeding sites. In the video, some specimens captured and sampled:

Monitoring also ended on the Biferno

On the Adriatic side, the substantial stability of the Biferno in recent seasons has favored a natural increase in the youth stadiums, which our technical staff has found in large numbers even at the mouth of those small spring streams that had been identified as suitable sites for the enhancement of reproductive capabilities. One above…

Completed the monitoring in the Volturno

The samplings for monitoring the results of the last breeding season in the Volturno river have ended. Now in the Unimol laboratories, the scientific staff is preparing to process the samples taken and to process the results of the genetic analyzes, ready in a few months: however, we can already anticipate something … On the…

The three types of behavior of male trout in the reproductive period

In the video and in the photo you can see two specimens of male trout while side by side they feed in peace, without fighting each other. This happens because they are not yet in the reproductive period, otherwise we would see them as rivals and fighters. In fact, it is known that in salmonids…

Autumn samples – Carpino stream

The genetic analyzes of the first year of the project had revealed, in some peripheral basins of the Volturno River, the presence of resident (non-migratory) populations with a discrete component of indigenous genetics. We are now continuing these investigations to discover, before the breeding season, the possible presence of pure source populations to be used…

Autumn Samples – Rio Santa Maria

In recent weeks we have carried out monitoring in the Biferno basin in Santa Maria del Molise (Rio Santa Maria), where we had placed artificial nests last spring. Here are some of the individuals from whom tissue samples were taken for DNA analysis. The numerous parr spots (narrow and elongated), typical of Mediterranean trout, bode…

Trout from Volturno 2020

Here are the trout from Volturno, the result of spring sowing and a lot of commitment and passion on the part of the project staff!

Work in progress in Rocchetta a Volturno.

The adaptation works in the hatchery of Rocchetta a Volturno proceed incessantly. In addition to the finalization of the hatchery, before the next breeding season there will also be an educational center with a multipurpose room, for conferences and face-to-face meetings that we hope to be able to organize as soon as we are in…

Acquatrekking always and anyway? No thanks!

We were inspired by a reflection by Alex Vigliani, an expert environmental hiking guide, to ask ourselves whether or not to practice this “sport” that is becoming popular in our territories. For those few who do not yet know it, it is a matter of practicing the walk not only on land, but in the…

Summer trout ascent in the Biferno

Slow motion emotion: in the tributary streams of the Biferno we managed to film some specimens in ascent! In fact, with the summer storms, the instinct of the ascent pushes the trout to explore the upper quadrants of the rivers and streams in which, in the winter months, they will reproduce.

The  parr spots

In the sampled specimens, in addition to genetic analyzes, morphological ones concerning 7 phenotypic classifications are also carried out, including the analysis and comparison of the so-called “parr spots”. It is a matter of observing 9-13 large ellipsoidal blue-grayish spots present along the median region of each side, sometimes divided or fragmented and / or…

The first fry of the 2020 season

The photo is not that great, but it is the first documented native Mediterranean trout fry, born in an artificial nest of the Rio Santa Maria, the result of fertilization with cryopreserved seed. The success of the sowing activities can only be assessed by monitoring the next few years, but in the meantime numerous fry…

Volturno trouts

One of our cages for the control of the breeding was positioned in the Rio Caprionero, because it is from there that the trout from the Volturno river go back to go to reproduce. In fact, the main course of the river, although it has a good substrate for reproduction, is vulnerable due to the…

Work starts again in Oratino

After more than two months of lockdown in which Italy had to stop due to the pandemic from Covid19, we finally start again with all due caution. Work is underway in Oratino to make the hatchery accessible to all, in the hope of reopening our facilities to visitors as soon as possible.

Rio Freddo trout (Biferno river basin)

The photo shows us a genetically pure individual of about 50 cm caught just downstream of the entrance of the Rio Freddo in the main course of the Biferno. In the samplings of this winter and early spring we observed that a part of the spawners in the Biferno River go up the Rio Freddo,…

The sowing of the larvae

Among the various methodologies provided by our project, there is also the sowing of the larvae hatched in the hatchery and taken a few days before free feeding to avoid domestication. Here we are in Rio Freddo and we have just built a nest with the addition of plants and mosses that will provide protection…

Nesting in Volturno river

Before sowing, we dedicated ourselves to the construction of nests in the main course and in the sides of the Volturno river. The main course of the Volturno river was heavily hit by the floods of last autumn-winter and, following the monitoring carried out, we recorded a severe depletion of the salmonid population. For this…

Nesting & Cocoonig: step by step a fundamental action of our project

5,000 native trout eggs from Molise, genotyped and fertilized with cryopreserved semen are ready to hatch, protected in their nests rebuilt directly in the rivers they belong to. Reintroducing embryonic eggs of S. magrostigma native trout into the nests is a key factor to support natural reproduction and to support the recolonization of the project…

Artificial fertilization of wild indigenous people: how does it work?

As we have already said elsewhere, the first step is to select the breeding stock. The specimens are captured, temporarily retained and genetically characterized (or recognized, if already marked). Male migration begins first, therefore the semen is taken first, which is put to freeze in the cryobank. Subsequently the eggs are taken from a certain…

The eggs are close to hatching!

It is spring and the embryonic S. macrostigma eggs, fertilized this winter partly with fresh seed, partly with frozen one, are close to hatching. For this reason we must hurry and bring them to their rivers: despite the pandemic, in fact, the NatSalMo technical staff – with all the necessary precautions and strictly observing the…

The protection of the cryobank continues

Even in this period of pandemic from Covid-19, in our project there are some invaluable actions that must absolutely be carried out in order not to risk nullifying the work done so far. One of these is the topping up of liquid nitrogen in the cryobank containers of the native Mediterranean trout seed, the first…

Oratino hatchery

In the municipality of Oratino, in the Bivaro area, the project staff recovered the old wash house by cleaning it and transforming it into an hatchery. The major obstacle to implementing this transformation was due to the impossibility of installing air conditioners to refrigerate the water necessary for optimal incubation of the eggs. But thanks…

Semen cryopreservation

One of the great innovations of our project is the creation of the first cryobank of the Mediterranean trout seed, a precious tool for the conservation and protection of the species. In January 2020, the project staff set up a mobile laboratory at the Rocchetta hatchery in Volturno to freeze the seminal material taken from…

Learning visit – day #2

At Biferno, Boiano locality. Cages are cleaned every day: in fact, together with natural waste, the current also carries a lot of waste, which must be removed as soon as possible to allow the trout in the cage to remain in the best possible conditions. Subsequently, we proceed to recover the trout rising from the…

NatSalMo project – Learning visit – day #1

February 13th, 2020, first day of the Meeting and Nicolaia Iaffaldano, project manager, immediately illustrates the transferability actions of the NatSalMo project to the Romanian colleagues who have come here from the universities of ULBS and UAIASI. The work is now proceeding quickly and dr. Gibertoni with dr. Esposito reveal interesting details that have emerged…

Learning visit on February 13th and 14th at UNIMOL

The next 13 and 14 February the whole project team will meet in Campobasso, at UNIMOL, for a training meeting with the Romanian delegation of the universities of ULBS and UAIASI. In the two days dedicated to the learning visit not only the project will be illustrated, but the progress of the work will be…

Morphological analysis and integration with genetic analysis

Also in the context of the preparatory actions for the project, the morphological analysis of the captured, labeled and photographed individuals was evaluated, from which biological tissue samples (fragment of fat fin) were taken for genetic analysis. The measurements and the counting of the livery characteristics are done through the ImageJ software. The morphological analysis,…

Barriers and pots for selective access installed on Rio Caprionero

The actions to restore and protect the populations of native S. macrostigma trout in the Volturno and Biferno basins continue with great alacrity. To promote the natural reproduction of native Mediterranean trout, we have just finished installing the barriers and pots for selective access to the main reproduction sites of the Volturno and Biferno. But…

Genetic analysis results on sampled individuals

As part of the preparatory action A3, the sampling stations involved 30 sites within the project area in order to cover a wide range of river habitats. The specimens, over 300 between males and females, were captured with electrofishing, measured by length and weight and marked by microchip (Pit-Tag) and with clearly visible T-anchor plates.…

River connectivity determination

For each flow, the Project team determined the river connectivity, or – by means of a visual census – the identification of those obstacles (natural or artificial barriers) that limit the movement and the fish lit. The sections of the waterways downstream of the 30 sampling sites of the chemical-physical analyzes and of the substrate…

The First Nat.Sal.Mo Open Forum

The first Open Forum of the Nat.Sal.Mo. project was held on 5 December in the conference room of the Molise Region in Campobasso. It was an information and training meeting aimed at the administrators and technicians of the local authorities involved in various capacities in the management and planning of the two river basins covered…

No Kill Zone signs delivered to CIPM

In the early morning of December 5th Nicolaia Iaffaldano, project manager of the NatSalMo project, together with the president of the CIPM association Giuseppe Mastrangelo and a large group of researchers and scholars, planted and inaugurated the signs useful for fishermen who will find themselves in the No Kill Zone of Biferno next fishing season.…

First Open Forum on December 5th

For our project on December 5th will be a day full of important events: in the morning our team of scholars will visit the breeding sites of Biferno and Volturno to take stock of the implementation of river connectivity. On that occasion we will also plant the No Kill Zone warning signs strongly desired by…

Water analysis: very comforting results

As anticipated, in the meeting held on 19 November at Unimol in the presence of the entire partnership, the results obtained so far from the actions carried out in the previous months were presented. In particular, the meeting opened with dr. Bruno Testa who illustrated the results of the analyzes conducted in the waters of…

Partnership meeting on 19th November 2019: a great success of attendance and content

The meeting on November 19th 2019 in which the entire partnership of the LIFE NatSalMo project met at the Agriculture Environment and Food Department of the University of Molise di Campobasso was a real success in terms of attendance and content. The representatives of UNIMOL, Mediterranean Trout Research Group (MTRG), Legambiente (LAM), Studio Giuliano (SG)…

The Life Nat.Sal.Mo project at the Life Ticino Biosource workshop

In a workshop dedicated to LIFE projects to protect fish fauna, Nat.Sal.Mo, on the recovery and protection of native Mediterranean trout in the Molise river basins, could not be missing. Representing our project on October 4th in Pontevecchio Magenta (MI) there was P.P. Gibertoni of MTRG, who masterfully illustrated to the numerous participants the numerous…

The first withdrawals with electrofishing

One of the first actions of our project involves sampling and genetic analysis on the Mediterranean trout populations currently present in the Volturno and Biferno. Last winter the first samples were taken with the electro-phishing technique: a narcosis is induced with calibrated electrical impulses and, after taking measurements and measurements, the specimen is released where…

Glossary in pills – Salmo Macrostigma

Let’s kick off a small in-depth section of the terminology used in the NatSalMo project. Salmo macrostigma Not everyone knows that the Mediterranean trout typical of central-southern and insular Italy has a scientific name, or rather, two: Salmo macrostigma or Salmo cettii. Today we tend to consider the two nomenclatur as synonyms, but in truth…

Conference in Sibiu- Very positive reception for NatSalMo project

In Sibiu a delegation of researchers from the LIFE NatsalMo project, led by Project Manager Nicolaia Iaffaldano, has disclosed the excellent results obtained so far in the most effective procedures to be applied to optimize the cryopreservation protocol of the sperm of the Salmo macrostigma . The welcome of the Romanian colleagues was, as always,…

International Conference on Aquatic Biodiversity in Transylvania

The first important results of the studies conducted within the Project will be presented by Nicolaia Iaffaldano and other scholars of the NatSalMo team at the International Conference on Aquatic Biodiversity – organized by the Lucian Blaga University – which will take place from 25th to 28th on September 2019 in Sibiu, Romania. The conference…

Newsletter no. 1

From La Nuova Ecologia: a brief piece about our project

“Molise: teachers and researchers working to save Mediterranean trout” The article La nuova Ecologia is available HERE

LIFE Nature and Biodiversity European Program: UniMol with the Agriculture, Environment and Food Department, leader and promoter of the LIFE Nat.Sal.Mo project

The first meeting of the Nat.Sal.Mo project – Recovery of Salmo macrostigma: Application of innovative techniques and participatory governance tools in rivers of Molise , funded under the European LIFE+ program was held. The University of Molise – with the Agriculture, Environment and Food Department – as well as being the promoter and leader of…

The project Nat.Sal.Mo presented in the Molise Region

THE PROJECT LIFE NatSalMo, a European project for the recovery of native trout in the Molise river basins, was presented today, May 28th at the Molise Region’s Department of Agricultural Policies and Environmental Protection. Nicola Cavaliere (Regional Councilor for Agricultural and Agri-food Policies, Rural Development, Forestry Planning, Hunting and Fishing, Productive Fishing, Environmental Protection, Soil…

LIFE Project Nat.Sal.Mo: the project for the recovery and conservation of native Mediterranean trout has just started